At 24 h after transfection, cell lysates were prepared and subjected to SDS-PAGE. UGC CUA CCA CCU U and GGA UUC GAA CUU ACA AUC A, respectively. The target sequence of siRNA for mouse and are: UGUUGCCUCUGUUCCCAUA and GCA CAU UCG UCA GGA AGA A, respectively. Silencer Select siRNAs were purchased from Life Technologies.(TIF) pone.0083639.s003.tif … Continue reading At 24 h after transfection, cell lysates were prepared and subjected to SDS-PAGE